Thursday, 18th February Lorne Infection and Immunity 2021

HIGH-THROUGHPUT SCREENING FOR DRUG AND BIOMARKER DISCOVERY: THE PRESENT AND THE FUTURE

Speaker: Dr Amee George & Shima Hamidi

Dr Amee George is a Research Fellow in the ACRF Department of Cancer Biology and Therapeutics and heads the ANU Centre for Therapeutic Discovery (ACTD), at the John Curtin School of Medical Research at The Australian National University in Canberra, Australia. She has a keen interest in high-throughput screening and the use of functional genomics-based approaches to investigate the molecular basis of disease. She leads screening-based research investigating diseases of the ribosome (ribosomopathies), in particular, the congenital bone marrow failure disorder Diamond Blackfan Anaemia (DBA), receiving funding from the Australian National Health and Medical Research Council (NHMRC), the Captain Courageous Foundation and Maddie Riewoldt’s Vision. Dr George also collaborates with national and international researchers from diverse medical research areas who wish to undertake drug and biomarker discovery projects.

This presentation will provide a broad overview of the technology and approaches for high-throughput screening, as well as provide some short vignettes into screening-related research. New screening approaches and examples for how this technology could assist in the infection and immunity research field will also be discussed.

• Pulmonary vaccines against Tuberculosis - effective and feasible - Warwick Britton
• How malaria parasites gain access to nutrients to facilitate their survival - Tania de Koning-Ward

• OPX: A new class of antibiotics for drug-resistant Gram-negative infections - Mark A. T. Blaskovich
• Repurposing a neurodegenerative drug to treat Gram-negative antibiotic resistant bacterial infection - David MP De Oliveira
• A unique glycosyltransferase effector from enteropathogenic Escherichia coli that targets innate immune signalling proteins - Cristina Giogha
• The molecular basis for zinc uptake via Streptococcus pneumoniae AdcAII - Marina Zupan

• Humoral Immune Responses To Plasmodium vivax Malaria: Application To Sero-Surveillance For Accelerated Malaria Elimination - Rhea Longley
• Evaluation of serosurveillance to understand the micro-heterogeneity of sub-microscopic malaria and other malaria risk factors in Western Cambodia. - Merryn Roe
• Defining transcriptome dynamics underlying memory CD4+ T cell development during malaria - Megan Soon
• High dimensional mass cytometry analysis dissects T cell and B cell heterogeneity in the immune response to Plasmodium vivax malaria and identifies signatures predicting immunity to infection - Lisa J Ioannidis

An opportunity for ECR's to join the National Invited Speakers with a virtual coffee 

• Regulation of NOD-like receptor signaling: role in colorectal cancer - Dana Philpott
• Immune responses mediated by bacterial membrane vesicles and their intracellular fate. - Maria Kaparakis Liaskos
• New drugs for superbugs: immunotherapeutics that enhance neutrophil clearance of Staphylococcus aureus - Jennifer A E Payne
• RIPK1: a master regulator of cellular immunity in protection against mucosal infection - Jaclyn S Pearson
• Mapping Autoantibody Responses in Children with Acute Rheumatic Fever - Reuben McGregor
• Rough and smooth variant Mycobacterium abscessus infections are differentially controlled by host immunity during chronic infection - Stefan H Oehlers
• Mutations in Streptococcus pneumoniae biosynthesis genes influence pneumococcal interactions with human respiratory epithelial cells and alter the epithelial-innate immune response - Caroline M Weight

Be sure to view the e-Poster Hall throughout the conference.  All e-Poster and Science Bite presenters will be online to answer any questions during both Poster Sessions.

• Developing a high-throughput multiplex immunoassay that characterises antibody responses to StrepA vaccine antigens - Alana L Whitcombe
• CD8+ T cell cross-reactivity across conserved Influenza A and B epitopes - Andrea Nguyen
• Age related epigenetic changes associated with and causative of intrinsic CD8+ T cell dysfunction - Daniel Thiele
• Humoral immune responses during acute bacterial urinary tract infection in mice - Sophia Hawas
• Modeling the immune complex cycling in follicular dendritic cells - Theinmozhi Arulraj
• Targeting SARS-CoV-2 using stealth nanoparticles loaded with siRNAs - Adi Idris
• A novel purification procedure for active recombinant human DPP4 and the inability of DPP4 to bind SARS-CoV-2 - Cecy R Xi
• SARS-CoV-2 infects but does not replicate in human macrophages and triggers pro-inflammatory and anti-viral cytokine responses. - Larisa I Labzin
• Antibodies to neutralizing epitopes synergistically block the interaction of the receptor binding domain of SARS-CoV-2 to ACE 2 - Manisha Pandey
• Structure of SARS-CoV-2 Nsp1/5′-UTR Complex and Implications for Potential Therapeutics, Vaccine & Virulence - Naveen Vankadari
• The alternative sigma factor RpoE2 is involved in the stress response to hypochlorite and in vivo survival of Haemophilus influenzae - Aidan Fletcher
• Molecular insights into secreted toxins from Escherichia coli pathogens reveal approaches to combat diarrheal infections                                                                                               - Akila Pilapitiya
• Hijacking of lipid synthesis and storage during flavivirus infection - Alice Trenerry
• Inferior outcomes in lung transplant recipients with serum Pseudomonas aeruginosa specific cloaking antibody - Amy Pham
• Genetic diversity and antibiotic resistance rates amongst recent Australian NTHi clinical isolates - Daniel Ellis
• Shape-shifting bacteria are key to infection - Elizabeth Peterson
• Spatiotemporal quantification of host membrane lipid order during Mycobacterium tuberculosis infection - George Ashdown
• H. influenzae nutritional virulence determines outcomes of interactions with human host cells during intra- and extracellular growth - Jennifer Hosmer
• Age-dependent innate and adaptive cellular immune responses in malaria-naive children and adults. - Jessica R Loughland
• Elucidating genes that are required for chronic infections of Salmonella enterica - Jessica L Rooke
• The Salmonella Effector SseK3 Targets Small Rab GTPases - Jiyao Gan
• Novel serum-resistance mechanism in patients with P. aeruginosa bacteraemia - Johannes Hoehensteiger
• Defining the glycointeractome of the multidrug resistant pathogen Acinetobacter baumannii: towards novel treatments and therapeutics - John M Atack
• Induction of stable human FOXP3⁺ Tregs by a parasite-derived TGF-β mimic - Laura Cook
• Antimicrobial activity of outer membrane vesicles against Gram‑negative and Gram‑positive bacteria is altered by their mechanism of biogenesis - Lauren Zavan
• Characterisation and inhibition of bacterial virulence factors - Lilian Hor
• Salmonella Typhimurium induces cIAP1 degradation to promote death in macrophages - Madeleine Wemyss
• 'Unleishing’ host cell death pathways to promote clearance of Leishmania donovani - Michelle P Clark
• Maurer’s cleft tethers – is tethering important for malarial adhesion? - Mohini A Shibu
• Host and pathogen genetic determinants that contribute to Buruli ulcer severity - Sachintha Wijegunasekara
• Complement and Fcγ-receptor mediated antibody effector functions target merozoites and are associated with protection from severe malaria in children - Sandra Chishimba
• Pathological and immune responses to chronic Mycoplasma gallisepticum infection and protective immunity of the ts-304 vaccine in chickens - Sathya N Kulappu Arachchige
• Characterization of Sec13 as a host-interactor in influenza using a Yeast-based system and Mammalian cells - Sonja Chua
• Super-resolved view of PfCERLI1, a rhoptry associated protein essential for Plasmodium falciparum merozoite invasion of erythrocytes - Sonja Frolich
• Drug addiction in the human malaria parasite plasmodium falciparum - Tayla Williamson
• Investigating the role of Plasmodium falciparum exported proteins that bind the new permeability pathway complex protein RhopH2 - Thorey Jonsdottir
• The gastrointestinal tract is a central site of dengue virus pathology and possible key to severe disease - Adriana Pliego
• T follicular helper (Tfh)-germinal centre (GC) B cell response is required for sterile immunity during enteric helminth infection - Aidil Zaini
• Depletion of alveolar macrophages by cigarette smoke causes delayed clearance of Legionella pneumophila infection - Garrett Z Ng
• Stabilization of tristetraprolin modulates inflammation during Mycobacterium tuberculosis infection - Giang Hoang Bao Le
• Functional cure of chronic hepatitis B is associated with co-occurrence of HBsAg/anti-HBs immune complex peaks with ALT flares, and seroconversion to potently neutralising anti-HBs - Hui Xu
• TLR4 signalling pathway in the gut attributes to the intestinal inflammation during dengue infection - Jaehyeon Kim
• Missense mutations in the gene encoding the cell death effector MLKL lead to lethal neonatal inflammation in mice and are present in high frequency in humans.  - Joanne M Hildebrand
• Neonatal sepsis: exploring the plasma proteome - Julie Hibbert
• Antimicrobial susceptibility of Staphylococcus aureus from different ocular conditions - Madeeha Afzal
• Synergistic activity of ciprofloxacin and azithromycin against staphylococci and streptococci - Manjulatha Mrs Sara
• Essential protective role for miR-652 during intracellular bacterial infection - Maxwell Stevens
• The unique type-I Interferon-epsilon (IFNε) constitutively protects the female reproductive tract from Zika Virus infection. - Rosa Coldbeck-Shackley
• PI3K gamma suppresses macrophage mediated inflammation in cystic fibrosis cells - Neeraj Tuladhar
• Monophasic Salmonella Typhimurium strains exhibit increased replication capacity in human macrophages by modulating intracellular host responses. - Rebecca Ambrose
• Simultaneous Induction of Cardiac and Neurobehavioral Autoimmune Pathology Following Exposure to Streptococcal Antigens - Rukshan AM Rafeek
• Screening for Chlamydia trachomatis infection in tubal ectopic pregnancy patients in India: Is serology the answer - Shipra Pant
• Investigating the impact of helminth infection on young and aged T_VM cells - Tabinda Hussain
• Histone deacetylase 7 promotes innate immune defence against bacterial infection, as well as Pkm2-dependent production of the monocyte recruiting chemokine Ccl2 - Yizhuo Wang
• Epithelial lytic cell death promotes monolayer permeability, apical elimination and lamellipodia-driven mechanism of epithelia resealing - Alexis Bonfim-Melo
• Therapeutic targeting of the myofibroblast NLRP3 inflammasome as a novel approach to treating fibrosis - Anita A Pinar
• Application of activity-based probes to interrogate the contribution of cathepsin X to dendritic cell function - Bangyan Xu
• MAIT cells expand in the absence of NKT and γδT cells  - Calvin Xu
• Live imaging of pyroptotic macrophages reveals novel GSDMD-dependent cytoskeletal remodelling - Caroline L Holley
• Activation of stimulator of interferon genes in dendritic cells induces interferon-lambda and subset- and species-specific dendritic cell death - Ee Shan Pang
• Viral Immune Evasion: There are more than two STATs - Gregory Moseley
• IL-37 employs multiple strategies to suppress inflammasome-mediated IL-1β bioactivity and inflammation - Ina Rudloff
• Bactericidal capacity of ascitic fluid from patients with decompensated cirrhosis - Isanka U Ratnasekera
• Exploring the MIF family of cytokines: Different roles for MIF and D-DT in inflammation - James Harris
• Tracking variability in the antimicrobial zinc toxicity response of human macrophages against Escherichia coli - Jessica B von Pein
•  WNT signatures are an integral part of macrophage responses to infection - Johanna K Ljungberg
• Dengue Infection Causes Membrane Recruitment of FH Through Ligands other than Glycosaminoglycans - Joshua G Dubowsky
• Enteric viral infections prior to gluten intake exacerbate type I interferon response through an RNA methylation dependent mechanism - Maialen Sebastian-delaCruz
• Interferon-lambda: a key cytokine for dendritic cell development? - Mariam Bafit
• The role of intracellular pattern recognition receptors in recognition and immunomodulation of dendritic cells in response to daptomycin resistant Staphylococcus aureus. - Nazneen Jahan
• Interferon epsilon alters peritoneal myeloid cell populations in a murine model of ovarian cancer - Nicole K. Campbell
• Development of a 3D cell culture model to study macrophage biology and infection with Leishmania mexicana - Sean W Cutter
• Unravelling mechanisms of TLR-inducible mitochondrial fission in macrophages - Syeda Farhana Afroz
• Characterizing a novel anti-inflammatory activity of Genistein - tomalika Ms ullah
• Differential modulation of innate immunity by Bacteroides fragilis bacteria and their secreted outer membrane vesicles   - William J Gilmore
• Lactic acid produced by an optimal vaginal microbiota promotes cervicovaginal epithelial barrier integrity: implications for HIV transmission - Brianna Jesaveluk
• The gut microbiome: A source of antimicrobial resistance for pathogens - Emily L Gulliver
• Identification of novel bacterial species associated with paediatric inflammatory bowel disease through direct mucosal sampling - Gemma L DAdamo
• Characterisation of host-microbiome interactions using microfluidic organ-on-a-chip models - Michelle Chonwerawong
• Functional Characterisation of Gastrointestinal Microbes - Remy Young
• Identification of suitable host conditions for the hatching of t muris t suis and t trichiura in the mucosal microbiota of pigs humans and a humanised mice model to understand the molecular basis of hatching in trichuris species - Tapoka T Mkandawire
• MicroRNA-16: Potential role in Chlamydia trachomatis-induced recurrent spontaneous abortion - Ankita Ray
• Is there potential clinical significance of miR-323-3p in Chlamydia trachomatis-associated tubal ectopic pregnancy? - Astha Dimri
• Disulphide bond dependent bacterial pathogenesis - Carlos Fernando Santos Martin
• Investigating molecular markers of Plasmodium falciparum artemisinin resistance in Papua New Guinea  - Dulcie Lautu
• Bacterial membrane vesicles: a potential reservoir for antimicrobial resistance genes within bacterial communities - Ella L Johnston
• Molecular mechanisms of autotransporters - Jason Paxman
• Genomic characteristics of exoU and exoS ocular Pseudomonas aeruginosa isolates - Mahjabeen Khan
• Identification of dengue virus (DENV) NS1 protein residues that are critical to its secretion - Brandon E.K. Tan
• Galectin-1 Plays a Significant Role in Preterm Birth in Women Infected with Ureaplasma urealyticum - Tanu Bhati
• Investigating the molecular evolution of carbapenem-resistance in Klebsiella quasipneumoniae - Natalia Rosas Bastidas
• Evolutionary Glycomics: A Comprehensive Study of Vertebrate Host Serum/Plasma Glycome Using Orthogonal Glycomics Techniques - Abarna V. M. Murugan
• Bioinformatics analysis of chemotaxis related genes in gram negative bacterial colonisers of the gastrointestinal tract  - Michael Bentley
• Understanding Plasmodium vivax antigen diversity and identifying targets of immune selection  - Paolo Bareng
• Single cell transcriptional profiles of immune cell subsets from children and adults with malaria. - Tinashe G Chabikwa
• Nanoparticle-mediated pulmonary delivery of the peptide hormone relaxin abrogates chronic allergic airways disease - Amlan Chakraborty
• Rapid profiling of polymyxin B susceptibility in gram-negative bacteria with a novel polymyxin-derived fluorescent probe - Bing Zhang
• Development of a Novel Antimalarial Class with a Slow Onset of Parasite Activity - Brodie L Bailey
• Genetic characterisation of zoonotic rotavirus strains identified in children and adults with acute rotavirus gastroenteritis in Australia  - Celeste M Donato
• Keep holding on: the role of antibody avidity in immunity to malaria - Jessica L Horton
• Investigation of the optimal strategy to deliver a B cell immunogen for a HCV vaccine. - Joey McGregor
• Intrapulmonary vaccination with delta-inulin adjuvant stimulates non-polarised chemotactic signalling and diverse cellular interaction - Kia C Ferrell
• Synchrotron-based X-ray fluorescence microscopy reveals accumulation of polymyxins in single human alveolar epithelial cells - Mohammad AK Azad
• Identification of estrogen receptor modulators as inhibitors of flavivirus infection - Nicholas S Eyre
• Fortrexo: New approach to prevent and treat viral infectious diseases  - Ranja Salvamoser
• Taming the beast: disarming pathogenic bacteria with novel anti-virulence agents - Geqing Wang
• Molecular surveillance over 14 years confirms reduction of Plasmodium vivax and falciparum transmission after implementation of Artemisinin-based combination therapy in Papua, Indonesia - Zuleima Pava
• Human Amniotic Epithelial Stem Cells attenuate Crescentic Glomerulonephritis - Ahmed Al Mushafi

• The role of the short chain fatty acid butyrate in CD4+ T cell immunity - Ariane Lee
• Characterization of a monoclonal antibody towards the N-terminal hypervariable region 1 (HVR1) and epitope I of Hepatitis C Virus Glycoprotein E2 - Felicia Schlotthauer
• Regulation of immune memory formation and function during viral infection - Lucy Cooper
• Liver resident CD4 T cell in malaria infection - Matthias H Enders
• B cells are required for optimal CD4⁺ T cell memory response against Salmonella infections - Meghanashree M Shreenivas
• Characterising interacting thymocytes and thymic stromal cells for mapping physical thymocyte-stroma interactions during T cell development - Yi Wang
• Optimizing a dual RNA-sequencing protocol for the analysis of host-pathogen interactions during neonatal sepsis - Isabella A. Joubert
• Therapeutic blockade of CXCR2 rapidly clears inflammation in Arthritis and Atopic Dermatitis models: Demonstration with surrogate and humanized antibodies - Md Jahangir Alam

• The role of Kelch 13 protein in the malaria parasite Plasmodium falciparum - Madel V Tutor
• Identifying novel Vgamma9Vddelta2 T cell phenotypes during human Plasmodium falciparum infection - Nicholas L Dooley
• The role of SMG1 in regulating innate immunity - Hui-Chi Lai
• The transmembrane TLR adaptor SCIMP scaffolds Erk1/2 to drive macrophage pro-inflammatory responses - Richard M Lucas
• Characterisation of a novel type I interferon pathway and its implications in inflammatory disease - Stephanie Huang
• The flexible usage of diverse cell death pathways ensures host protection against Salmonella Typhimurium infection - Sven Engel
• Intestinal microfold cells orchestrate the interactions between microbiota and immunity - Wang Cao
• Structural and functional heterogeneity in pathoadaptive FimH adhesin variants in Escherichia coli - Jilong Qin
• An integrated analysis of myeloid cells identifies gaps in in vitro models of in vivo biology - Nadia Rajab
• Global 3'-UTR length changes mediated by interferon beta in murine and human macrophages - Sarah Straub

• A role of C. albicans Rpn4 in regulating antifungal drug susceptibility - Ka Pui Sharon Yau
• The experimental evolution of antibiotic resistance reveals chinks in the armor of multidrug-resistant pathogens. - Michael McDonald
• Solving bottlenecks and providing new strategies for AMR crisis - Nassos Typas